Gene Cloning, DNA Sequencing, and Expression of Thermostable β-Mannanase from Bacillus stearothermophilus
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چکیده
منابع مشابه
Gene Cloning, DNA Sequencing, and Expression of Thermostable b-Mannanase from Bacillus stearothermophilus
A DNA genomic library constructed from Bacillus stearothermophilus, a gram-positive, facultative thermophilic aerobe that secretes a thermostable b-mannanase, was screened for mannan hydrolytic activity. Recombinant b-mannanase activity was detected on the basis of the clearing of halos around Escherichia coli colonies grown on a dye-labelled substrate, Remazol brilliant blue-locust bean gum. T...
متن کاملGene cloning, DNA sequencing, and expression of thermostable beta-mannanase from Bacillus stearothermophilus.
A DNA genomic library constructed from Bacillus stearothermophilus, a gram-positive, facultative thermophilic aerobe that secretes a thermostable beta-mannanase, was screened for mannan hydrolytic activity. Recombinant beta-mannanase activity was detected on the basis of the clearing of halos around Escherichia coli colonies grown on a dye-labelled substrate, Remazol brilliant blue-locust bean ...
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The structural gene for a thermostable alpha-amylase from Bacillus stearothermophilus was cloned in plasmids pTB90 and pTB53. It was expressed in both B. stearothermophilus and Bacillus subtilis. B. stearothermophilus carrying the recombinant plasmid produced about fivefold more alpha-amylase (20.9 U/mg of dry cells) than did the wild-type strain of B. stearothermophilus. Some properties of the...
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A thermostable pullulanase (alpha-dextrin 6-glucanohydrolase [EC 3.2.1.41]) from a newly isolated Bacillus stearothermophilus strain (TRS128) was purified and characterized. The enzyme hydrolyzed (1-->6)-alpha-d-glucosidic linkages of pullulan to produce maltotriose, and the optimum temperature was 65 degrees C. About 90% of the enzyme activity was retained after treatment at 65 degrees C for 6...
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MANB36, a secrete endo-1,4-d-mannanase produced by Bacillus subtilis B36, was purified to homogeneity from a culture supernatant and characterized. The optimum pH value for the mannanase activity of MANB36 is 6.4 and the optimum temperature is 50 ∞C. The enzyme activity of MANB36 is remarkably thermostable at 60 ∞C and the specific activity of MANB36 is 927.84 U/mg. Metal cations (except Hg2+ a...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1998
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.64.11.4428-4432.1998